Difference between revisions of "Part:BBa K174004"
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<partinfo>BBa_K174004 short</partinfo> | <partinfo>BBa_K174004 short</partinfo> | ||
| − | This promoter can be induced by IPTG. Wild type | + | This promoter can be induced by IPTG. Wild type lac operator is replaced with a perfect palindromic "oid" operator sequence which increases the repression by LacI more than 2.5 fold. To repress the promoter LacI needs to be expressed. Addition of IPTG will derepress LacI activity. |
| − | This biobrick is an enhancement of Biobrick BBa_K090501 which has the sequence from pmutin2. | + | This biobrick is an enhancement of Biobrick BBa_K090501 which has the sequence from pmutin2. BBa_K143015 and BBa_I746665 biobricks have also the wild type lac operator. |
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Revision as of 15:01, 10 October 2009
Pspac promoter
This promoter can be induced by IPTG. Wild type lac operator is replaced with a perfect palindromic "oid" operator sequence which increases the repression by LacI more than 2.5 fold. To repress the promoter LacI needs to be expressed. Addition of IPTG will derepress LacI activity.
This biobrick is an enhancement of Biobrick BBa_K090501 which has the sequence from pmutin2. BBa_K143015 and BBa_I746665 biobricks have also the wild type lac operator.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]