Difference between revisions of "Part:BBa K4204004:Design"

 
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===Design Notes===
 
===Design Notes===
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The sequence is designed to replace the promotor, RBS, and ORF of the original repressor. Thus it does not have a terminator and the original terminator of the repressor must be kept. Also, the promoter of the sequence might be shorter than marked, since the original research does not mark the promoter range clearly.  
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The sequence is designed to replace the promotor, RBS, and ORF of the original repressor. The terminator is added artificially later. Also, the promoter of the sequence might be shorter than marked, since the original research does not mark the promoter range clearly.
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−
 
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===Source===
 
===Source===
  
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From research done by Ruegg et al [1]. Synthesized by twist bio.  
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From research done by Ruegg et al [1]. Synthesized by twist bioscience.  
  
 
===References===
 
===References===
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[1] Ruegg, T. L., Pereira, J. H., Chen, J. C., DeGiovanni, A., Novichkov, P., Mutalik, V. K., Tomaleri, G. P., Singer, S. W., Hillson, N. J., Simmons, B. A., Adams, P. D., & Thelen, M. P. (2018). Jungle Express is a versatile repressor system for tight transcriptional control. Nature Communications, 9(1). https://doi.org/10.1038/s41467-018-05857-3
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Latest revision as of 14:35, 11 October 2022


Transcriptional unit of EilR.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 475
    Illegal AgeI site found at 625
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence is designed to replace the promotor, RBS, and ORF of the original repressor. The terminator is added artificially later. Also, the promoter of the sequence might be shorter than marked, since the original research does not mark the promoter range clearly.

Source

From research done by Ruegg et al [1]. Synthesized by twist bioscience.

References

[1] Ruegg, T. L., Pereira, J. H., Chen, J. C., DeGiovanni, A., Novichkov, P., Mutalik, V. K., Tomaleri, G. P., Singer, S. W., Hillson, N. J., Simmons, B. A., Adams, P. D., & Thelen, M. P. (2018). Jungle Express is a versatile repressor system for tight transcriptional control. Nature Communications, 9(1). https://doi.org/10.1038/s41467-018-05857-3

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