Difference between revisions of "Part:BBa K4156107"
 
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We designed pPepT-Bxb1-φ174E to test the expression efficiency of φ174E under the control of a logic gate linking the pPepT and Bxb1 We will validate the function of this biobrick by measuring the viability of the bacteria.
 
We designed pPepT-Bxb1-φ174E to test the expression efficiency of φ174E under the control of a logic gate linking the pPepT and Bxb1 We will validate the function of this biobrick by measuring the viability of the bacteria.
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Latest revision as of 13:33, 11 October 2022


pPepT-Bxb1-φ174E

pPepT-Bxb1-φ174E is a composite part that express lysis gene φ174E, constructed with hypoxia-sensing promoter pPepT and serine integrase Bxb1.


Usage and Biology

We designed pPepT-Bxb1-φ174E to test the expression efficiency of φ174E under the control of a logic gate linking the pPepT and Bxb1 We will validate the function of this biobrick by measuring the viability of the bacteria.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 360
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 634
    Illegal XhoI site found at 721
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1468