Difference between revisions of "Part:BBa K4156106"
 
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pPepT-Bxb1 consists of a fusion of the pH-sensitive promoter pPepT and the serine integrase Bxb1. It will act as a complex regulatory for controlling downstream logic gates and transcription of genes.
 
pPepT-Bxb1 consists of a fusion of the pH-sensitive promoter pPepT and the serine integrase Bxb1. It will act as a complex regulatory for controlling downstream logic gates and transcription of genes.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 13:33, 11 October 2022


pPepT-Bxb1

pPepT-Bxb1 is constructed with hypoxia-sensing promoter pPepT and serine integrase Bxb1.


Usage and Biology

pPepT-Bxb1 consists of a fusion of the pH-sensitive promoter pPepT and the serine integrase Bxb1. It will act as a complex regulatory for controlling downstream logic gates and transcription of genes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 360
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 634
    Illegal XhoI site found at 721
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1468