Difference between revisions of "Part:BBa K4461013"
 
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<partinfo>BBa_K4461013 short</partinfo>
 
<partinfo>BBa_K4461013 short</partinfo>
  
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An improved alternative to mCherry (BBa_J18932) that reduces the fluorescence intensity by fusion with tag(BBa_K1051207).  
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An improved alternative to mCherry (
 +
<html>
 +
<a href="https://parts.igem.org/Part:BBa_J18932">BBa_J18932</a>
 +
</html>
 +
) that reduces the fluorescence intensity by fusion with tag(
 +
<html>
 +
<a href="https://parts.igem.org/Part:BBa_K1051207">BBa_K1051207</a>
 +
</html>
 +
).  
  
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We synthesized a commercial sequence of tag (BBa_K1051207) and EcoRI and HindIII cutting sites on its prefix and suffix. Therefore, we can insert the tag into our construct (BBa_K4461010) by restriction enzyme digestion.
+
We synthesized a commercial sequence of tag (
 +
<html>
 +
<a href="https://parts.igem.org/Part:BBa_K1051207">BBa_K1051207</a>
 +
</html>
 +
) and EcoRI and HindIII cutting sites on its prefix and suffix. Therefore, we can insert the tag into our construct (
 +
<html>
 +
<a href="https://parts.igem.org/Part:BBa_K4461010">BBa_K4461010</a>
 +
</html>
 +
) by restriction enzyme digestion.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 13:14, 11 October 2022


mCherry+tag fusion protein

An improved alternative to mCherry ( BBa_J18932 ) that reduces the fluorescence intensity by fusion with tag( BBa_K1051207 ).

We synthesized a commercial sequence of tag ( BBa_K1051207 ) and EcoRI and HindIII cutting sites on its prefix and suffix. Therefore, we can insert the tag into our construct ( BBa_K4461010 ) by restriction enzyme digestion.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 694
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 694
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 694
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 694
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 694
  • 1000
    COMPATIBLE WITH RFC[1000]