Difference between revisions of "Part:BBa K228005"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K228005 short</partinfo>
 
<partinfo>BBa_K228005 short</partinfo>
  
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It generates AraC regulatory protein which binds to and repress the pBad promoter(such as BBa_I13453). L-arabinose, which acts as inducer, could efficiently prevent the protein binding to pBad.
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This part contains AraC protein generator(<partinfo>BBa_K228007</partinfo>) and Pbad promoter(<partinfo>BBa_I13453</partinfo>). The expression of any coding sequence following the Pbad promoter is repressed constitutively by AraC protein and induced by L-arabinose.
  
 
<!-- Add more about the biology of this part here
 
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Latest revision as of 05:44, 9 October 2009

AraC protein and Pbad promoter

This part contains AraC protein generator(BBa_K228007) and Pbad promoter(BBa_I13453). The expression of any coding sequence following the Pbad promoter is repressed constitutively by AraC protein and induced by L-arabinose.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1274
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1214
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]