Difference between revisions of "Part:BBa K4151017"

 
 
Line 3: Line 3:
 
<partinfo>BBa_K4151017 short</partinfo>
 
<partinfo>BBa_K4151017 short</partinfo>
  
−
It can form a Tet-on system with seven or eight repeats of TetO.
+
Since AID is a deaminase that would induce point mutation, the expression of AID should be tightly regulated. We incorporate the Tet-on system, an inducible protein expression system, into our design to achieve this purpose. When tetracycline is added, it combines with rtTA(reverse tetracycline-controlled transactivator) to form a rtTA-tetracycline complex, which can recognize and attach to the TetO sequence and express our target protein. By this approach, our construct can regulate the amount of AID expressed.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 12:16, 11 October 2022


rtTA

Since AID is a deaminase that would induce point mutation, the expression of AID should be tightly regulated. We incorporate the Tet-on system, an inducible protein expression system, into our design to achieve this purpose. When tetracycline is added, it combines with rtTA(reverse tetracycline-controlled transactivator) to form a rtTA-tetracycline complex, which can recognize and attach to the TetO sequence and express our target protein. By this approach, our construct can regulate the amount of AID expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4
    Illegal NgoMIV site found at 621
  • 1000
    COMPATIBLE WITH RFC[1000]