Difference between revisions of "Part:BBa K238003"

Latest revision as of 12:42, 8 October 2009

Vanillin receptor

VirA/G system is a phenol sensing system originally found in agrobacterium tumefaciens. it binds phenols such as vanillin and the activates gene transcription.

VirA and RpoA both have illegal restriction sites in their original sequence. VirA has two PstI sites and RpoA two EcoRI sites. We used site directed mutagenesis to get these sites out. PstI was mutated from CTGCAG to CTGCAA. EcoRI was mutated from GAATTC to GAATTT.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 564
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 728
Illegal BglII site found at 1621
Illegal BglII site found at 4394
Illegal BamHI site found at 3287
Illegal XhoI site found at 2841
Illegal XhoI site found at 2934
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1467
Illegal NgoMIV site found at 4367
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1239
Illegal BsaI site found at 2031
Illegal BsaI.rc site found at 4023
Illegal SapI site found at 1713
Illegal SapI.rc site found at 942

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 __NOTOC__
 <partinfo>BBa_K238003 short</partinfo>
-this receptor is composed of a PBP that binds the vanilla. this complex then binds to the Trg-EnvZ fusion in the membrane. this fusion works intracellular through the ompR transcriptional factor.
+VirA/G system is a phenol sensing system originally found in agrobacterium tumefaciens. it binds phenols such as vanillin and the activates gene transcription.
+VirA and RpoA both have illegal restriction sites in their original sequence. VirA has two PstI sites and RpoA two EcoRI sites. We used site directed mutagenesis to get these sites out. PstI was mutated from CTGCAG to CTGCAA. EcoRI was mutated from GAATTC to GAATTT.
 <!-- Add more about the biology of this part here