Difference between revisions of "Part:BBa K4156084"
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HlyE is a toxin, by breaking the cell membrane of the tumor, the tumor cells are destroyed. In our experiments, we used HlyE as a tumor treatment factor and it is connected with the logic gate module and is subject to logic gate control expression.
 
HlyE is a toxin, by breaking the cell membrane of the tumor, the tumor cells are destroyed. In our experiments, we used HlyE as a tumor treatment factor and it is connected with the logic gate module and is subject to logic gate control expression.
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===Characterization===
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We use HlyE as one of the therapeutic proteins. Characterization details can be found at<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156119"> BBa_K4156119 </a></html> ; <html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156128"> BBa_K4156128 </a></html>.
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 11:44, 11 October 2022


HlyE

HlyE, fully known as hemolysin E, is encoded by hlyE of E. coli, which has been tested as a pore-forming antitumor toxin.


Usage and Biology

HlyE is a toxin, by breaking the cell membrane of the tumor, the tumor cells are destroyed. In our experiments, we used HlyE as a tumor treatment factor and it is connected with the logic gate module and is subject to logic gate control expression.

Characterization

We use HlyE as one of the therapeutic proteins. Characterization details can be found at BBa_K4156119  ; BBa_K4156128 .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 72
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]