Difference between revisions of "Part:BBa K4229018"
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<partinfo>BBa_K4229018 short</partinfo> | <partinfo>BBa_K4229018 short</partinfo> | ||
| − | + | These are the genes we put into MCS2. The first one is XiaI fused with the spycatcher(BBa_K4229015) and the second is the L-tryptophan transport protein TnaB(BBa_K4229003). It was decided to put the enzymes with the catcher at the beginning of each MCS as those are our most important proteins, which we wanted to have produced the most. These two genes build together with the Biobrick BBa_K42216 all enzymes of the indigo/indirubin pathway. The production was tested under the T7 promotor and LacI operator in those two Biobricks: BBa_K4229033 and BBa_K4229034) | |
| + | [[File:Grafik indigo weier bg.png|900px|thumb|left|]] | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 11:43, 11 October 2022
RBS-spyCatcherXiaI-RBS-TnaB
These are the genes we put into MCS2. The first one is XiaI fused with the spycatcher(BBa_K4229015) and the second is the L-tryptophan transport protein TnaB(BBa_K4229003). It was decided to put the enzymes with the catcher at the beginning of each MCS as those are our most important proteins, which we wanted to have produced the most. These two genes build together with the Biobrick BBa_K42216 all enzymes of the indigo/indirubin pathway. The production was tested under the T7 promotor and LacI operator in those two Biobricks: BBa_K4229033 and BBa_K4229034)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 893
Illegal AgeI site found at 783 - 1000COMPATIBLE WITH RFC[1000]