Difference between revisions of "Part:BBa K4245011"

 
 
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This part is the sequence for hsa-miR-208a-3p, an miRNA isolated from <i>Homo sapiens</i>. This miRNA acts as an upregulated biomarker for coronary artery disease (Kaur et al., 2020), and is therefore potentially useful for the early detection of this condition. hsa-miR-208a-3p, or miRNA-208a-3p, is seen to be related to the progression of CAD by regulating the proliferation of hVSMCs (Human vascular smooth muscle cells), which are crucial in the progression of coronary artery disease, via downregulation of BTG (Wang & Yan et. al., 2022). Thus, this biomarker increases the sensitivity of our biosensors by adding another component that can track the prognosis of the disease. As such, this miRNA can indicate how far along someone is in the development of coronary artery disease.
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The Lambert_GA 2022 team developed a set of padlock probes to use the rolling circle amplification approaches for several miRNAs related to CAD. This miRNA is used as the basis for <partinfo>BBa_K4245105</partinfo> and <partinfo>BBa_K4245112</partinfo>, the 3' arm for hsa-miR-208a-3p and 5' arm for hsa-miR-208a-3p, and as the target sequence for <partinfo>BBa_K4245207</partinfo>  and  <partinfo>BBa_K4245208</partinfo>  the hsa-miR-208a-3p RCA Padlock Probe and the hsa-miR-208a-3p RCT Padlock Probe.
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When using rolling circle amplification (RCA), the miRNA binds to the padlock. A rolling circle product (RCP) is produced from <partinfo>BBa_K4245131</partinfo> (Middle Sequence), which is then detected by the linear probes <partinfo>BBa_K4245130</partinfo> (Fluorophore) and <partinfo>BBa_K4245132</partinfo> (Quencher). When these parts bind to the RCP, the fluorescence decreases. Therefore, lower fluorescence is indicative of greater miRNA concentrations.
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<b>References:</b>
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Kaur, A., Mackin, S. T., Schlosser, K., Wong, F. L., Elharram, M., Delles, C., Stewart, D. J., Dayan, N., Landry, T., & Pilote, L. (2020). Systematic review of microRNA biomarkers in acute coronary syndrome and stable coronary artery disease. Cardiovascular research, 116(6), 1113–1124. https://doi.org/10.1093/cvr/cvz302
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Wang, D., & Yan, C. (2022). MicroRNA-208a-3p participates in coronary heart disease by regulating the growth of hVSMCs by targeting BTG1. Experimental and therapeutic medicine, 23(1), 71. https://doi.org/10.3892/etm.2021.10994
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 11:27, 11 October 2022

hsa-miR-208a-3p

This part is the sequence for hsa-miR-208a-3p, an miRNA isolated from Homo sapiens. This miRNA acts as an upregulated biomarker for coronary artery disease (Kaur et al., 2020), and is therefore potentially useful for the early detection of this condition. hsa-miR-208a-3p, or miRNA-208a-3p, is seen to be related to the progression of CAD by regulating the proliferation of hVSMCs (Human vascular smooth muscle cells), which are crucial in the progression of coronary artery disease, via downregulation of BTG (Wang & Yan et. al., 2022). Thus, this biomarker increases the sensitivity of our biosensors by adding another component that can track the prognosis of the disease. As such, this miRNA can indicate how far along someone is in the development of coronary artery disease.

The Lambert_GA 2022 team developed a set of padlock probes to use the rolling circle amplification approaches for several miRNAs related to CAD. This miRNA is used as the basis for BBa_K4245105 and BBa_K4245112, the 3' arm for hsa-miR-208a-3p and 5' arm for hsa-miR-208a-3p, and as the target sequence for BBa_K4245207 and BBa_K4245208 the hsa-miR-208a-3p RCA Padlock Probe and the hsa-miR-208a-3p RCT Padlock Probe.

When using rolling circle amplification (RCA), the miRNA binds to the padlock. A rolling circle product (RCP) is produced from BBa_K4245131 (Middle Sequence), which is then detected by the linear probes BBa_K4245130 (Fluorophore) and BBa_K4245132 (Quencher). When these parts bind to the RCP, the fluorescence decreases. Therefore, lower fluorescence is indicative of greater miRNA concentrations.

References:

Kaur, A., Mackin, S. T., Schlosser, K., Wong, F. L., Elharram, M., Delles, C., Stewart, D. J., Dayan, N., Landry, T., & Pilote, L. (2020). Systematic review of microRNA biomarkers in acute coronary syndrome and stable coronary artery disease. Cardiovascular research, 116(6), 1113–1124. https://doi.org/10.1093/cvr/cvz302 Wang, D., & Yan, C. (2022). MicroRNA-208a-3p participates in coronary heart disease by regulating the growth of hVSMCs by targeting BTG1. Experimental and therapeutic medicine, 23(1), 71. https://doi.org/10.3892/etm.2021.10994

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]