Difference between revisions of "Part:BBa K4239002"
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<p><i>fiatluxD</i> is made to be used with | <p><i>fiatluxD</i> is made to be used with | ||
| − | <i>fiatluxC</i> <a href="https://parts.igem.org/Part: | + | <i>fiatluxC</i> <a href="https://parts.igem.org/Part:BBa_K4239001" class="pr-0" target="_blank">(BBa_K4239001)</a> |
| − | and <i>fiatluxE</i> <a href="https://parts.igem.org/Part: | + | and <i>fiatluxE</i> <a href="https://parts.igem.org/Part:BBa_K4239005" class="pr-0" target="_blank">(BBa_K4239005)</a>. |
It codes for a subpart fatty acid reductase. With the subparts coding from <i>fiatluxC</i> and <i>fiatluxE</i>, they form a complex that recycles fatty acids to fatty aldehydes. Fatty aldehydes will be used as a substrat for the luciferase protein.</p> | It codes for a subpart fatty acid reductase. With the subparts coding from <i>fiatluxC</i> and <i>fiatluxE</i>, they form a complex that recycles fatty acids to fatty aldehydes. Fatty aldehydes will be used as a substrat for the luciferase protein.</p> | ||
<p>The systeme <i>fiatluxC/fiatluxD/fiatluxE</i> is made to be used with | <p>The systeme <i>fiatluxC/fiatluxD/fiatluxE</i> is made to be used with | ||
| − | <i>fiatluxA</i> <a href="https://parts.igem.org/Part: | + | <i>fiatluxA</i> <a href="https://parts.igem.org/Part:BBa_K4239003" class="pr-0" target="_blank">(BBa_K4239003)</a> |
| − | and <i>fiatluxB</i> <a href="https://parts.igem.org/Part:BBa_K239004" class="pr-0" target="_blank">( | + | and <i>fiatluxB</i> <a href="https://parts.igem.org/Part:BBa_K239004" class="pr-0" target="_blank">(BBa_K4239004)</a>, |
| − | gathered in the <i>fiatluxCDABE</i> <a href="https://parts.igem.org/Part: | + | gathered in the <i>fiatluxCDABE</i> <a href="https://parts.igem.org/Part:BBa_K4239008" class="pr-0" target="_blank">(BBa_K4239008)</a> |
operon.</p> | operon.</p> | ||
Revision as of 11:16, 11 October 2022
Enhanced luciferase subunits fiatluxD
Description
fiatluxD is made to be used with fiatluxC (BBa_K4239001) and fiatluxE (BBa_K4239005). It codes for a subpart fatty acid reductase. With the subparts coding from fiatluxC and fiatluxE, they form a complex that recycles fatty acids to fatty aldehydes. Fatty aldehydes will be used as a substrat for the luciferase protein.
The systeme fiatluxC/fiatluxD/fiatluxE is made to be used with fiatluxA (BBa_K4239003) and fiatluxB (BBa_K4239004), gathered in the fiatluxCDABE (BBa_K4239008) operon.
Fiatlux genes come from ilux genes (C, D, A, B, E). They were modified to remove every Igem restriction site (EcoR1, Xba1, Spe1 and Pst1) included in genes. They were also adapted to include the biobrick format.
The ilux operon was born from a mutated natural luminescence operon present in the bacteria P.luminescens: the lux operon. These mutations were error-prone PCR induced according to Gregor et al.’s study in 2018 (Gregor et al. 2018). The aim was to create a system of genes that produced more light than the lux system.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Construction
fiatluxC was directly synthesized with fiatluxD and the promoter J23117 (BBa_J23117). The description of the synthesis is on the following page part fiatluxCD (BBa_K4239006).
References
Gregor C, Gwosch KC, Sahl SJ, Hell SW. Strongly enhanced bacterial bioluminescence with the ilux operon for single-cell imaging. Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):962-967. doi: 10.1073/pnas.1715946115. Epub 2018 Jan 16. PMID: 29339494; PMCID: PMC5798359.