Difference between revisions of "Part:BBa K4165238"
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                             the concentration of the protein compared with the wet lab results.
 
                             the concentration of the protein compared with the wet lab results.
 
===WetLab Results===
 
===WetLab Results===
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<p style=" font-weight: bold; font-size:14px;"> Transformation of His UBE2V2 in BL-21 using pGS-21a vector </p>
 
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<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/wetlab-results/ube2v2-pgs.jpg" style="margin-left:200px;" alt="" width="500" /></p>
 
<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/wetlab-results/ube2v2-pgs.jpg" style="margin-left:200px;" alt="" width="500" /></p>
 
</html>
 
</html>
 
                                   Figure 2. Transformed plate of His UBE2v2 + pGS-21a  
 
                                   Figure 2. Transformed plate of His UBE2v2 + pGS-21a  
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<p style=" font-weight: bold; font-size:14px;"> Transformation of His UBE2V2 in DH-5 alpha </p>
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<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/wetlab-results/ube2v2-pjet.jpg" style="margin-left:200px;" alt="" width="500" /></p>
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                                  Figure 3. Transformed playe of His UBE2V2 + pJET
 +
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K4165238 parameters</partinfo>
 
<partinfo>BBa_K4165238 parameters</partinfo>
 
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Revision as of 11:03, 11 October 2022


His-UBE2V2

This biobrick consists of T7 promotor (BBa_K3633015), RBS (BBa_K4165016), 6-His tag (BBa_K4165020), UBE2V2 (BBa_K4165013), T7 Terminator (BBa_K731721), The His tag was attached to the UBE2V2 coding sequence to serve in the purification using NI-NTA column.

Source

Synthesized

Usage and Biology

Ubiquitin-conjugating E2 ligase has a role in the ubiquitination cascade for protein degradation. It has been used in this project to target and degrade both tau and Aβ proteins which are both considered the main causes of Alzheimer’s Disease pathogenesis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Dry lab

Mathematical modeling

Transcription rate and translation rate under T7 promotor

the mathematical modeling was based on our code for the calculation of transcription and translation (you can find it in the code section) beside with the estimated results from the wet lab. 

                  Figure 1. this figure shows the results from the transcription and translation code showing the 
                           the concentration of the protein compared with the wet lab results.

WetLab Results

Transformation of His UBE2V2 in BL-21 using pGS-21a vector 

                                  Figure 2. Transformed plate of His UBE2v2 + pGS-21a

Transformation of His UBE2V2 in DH-5 alpha 

                                 Figure 3. Transformed playe of His UBE2V2 + pJET