Difference between revisions of "Part:BBa K4361300"
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<h3>Usage and Biology</h3> | <h3>Usage and Biology</h3> | ||
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<figcaption> <b>Figure 1.</b> SDS PAGE gel of PURE reactions with GreenLys (fluorescent Cy2-labeled lysine) and a variant of </html>[[Part:BBa_K4361106]]<html> containing a BlcR mutant (this part through </html>[[Part:BBa_K4361319]]<html>). This part corresponds to lane <u>1</u>.</figcaption> | <figcaption> <b>Figure 1.</b> SDS PAGE gel of PURE reactions with GreenLys (fluorescent Cy2-labeled lysine) and a variant of </html>[[Part:BBa_K4361106]]<html> containing a BlcR mutant (this part through </html>[[Part:BBa_K4361319]]<html>). This part corresponds to lane <u>1</u>.</figcaption> | ||
</figure> | </figure> | ||
Revision as of 10:51, 11 October 2022
BlcR D37R
A mutant of the BlcR protein, created through site-directed mutagenesis with primers R1 (Part:BBa_K4361200) and F1.1 D37R (Part:BBa_K4361201). For this mutant, the aspartic acid in position 37 has been changed to arginine by mutating the GAC codon to CGC.
This mutant also contains the following nucleotide mutations outside of the targeted site:
- G 763 > A, resulting in substitution E255K
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 694
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 78
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 589
Usage and Biology
