Difference between revisions of "Part:BBa K4361300"
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<span class='h3bb'>Sequence and Features</span>
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<h3>Usage and Biology</h3>
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<figure>
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<a href="https://static.igem.wiki/teams/4361/wiki/results/greenlys.png"><img src="https://static.igem.wiki/teams/4361/wiki/results/greenlys.png" style="width:300px;margin-left:275px"></a>
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<figcaption> <b>Figure 1.</b> SDS PAGE gel of PURE reactions with GreenLys (fluorescent Cy2-labeled lysine) and a variant of </html>[[Part:BBa_K4361106]]<html> containing a BlcR mutant (this part through </html>[[Part:BBa_K4361319]]<html>). This part corresponds to lane <u>1</u>.</figcaption>
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Revision as of 10:50, 11 October 2022


BlcR D37R

A mutant of the BlcR protein, created through site-directed mutagenesis with primers R1 (Part:BBa_K4361200) and F1.1 D37R (Part:BBa_K4361201). For this mutant, the aspartic acid in position 37 has been changed to arginine by mutating the GAC codon to CGC.

This mutant also contains the following nucleotide mutations outside of the targeted site:

  • G 763 > A, resulting in substitution E255K

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 694
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 78
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 589

Usage and Biology

<figure> <a href="greenlys.png"><img src="greenlys.png" style="width:300px;margin-left:275px"></a> <figcaption> Figure 1. SDS PAGE gel of PURE reactions with GreenLys (fluorescent Cy2-labeled lysine) and a variant of </html>Part:BBa_K4361106 containing a BlcR mutant (this part through Part:BBa_K4361319). This part corresponds to lane 1.