Difference between revisions of "Part:BBa K4226003"
 
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<partinfo>BBa_K4226003 short</partinfo>
 
<partinfo>BBa_K4226003 short</partinfo>
  
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This part is an improvement for the part：[[Part:BBa_K1014002|Promoter hrpL]] uploaded by iGEM13_HIT-Harbin. This promoter will be active,when both hrpR ([[Part:BBa K4226001|hrpR Gene]]), and hrpS gene ([[Part:BBa K4226002|hrpS Gene]]), are present. The hrpL Promoter is transcriptionally upregulated by hrpR and hrpS. The Amp30E Amplification Device consists of hrpR ([[Part:BBa K4226001|hrpR Gene]]), hrpS ([[Part:BBa K4226002|hrpS Gene]]) and hrpL promoter.
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This part is an improvement for the part：[[Part:BBa_K1014002|Promoter hrpL]] uploaded by iGEM13_HIT-Harbin. This promoter will be active, when both [[Part:BBa K4226001|hrpR Gene]] and [[Part:BBa K4226002|hrpS Gene]] are present. The hrpL Promoter is transcriptionally upregulated by hrpR and hrpS. The [[Part:BBa_K4226004|Amp30E Amplification Device]] consists of [[Part:BBa K4226001|hrpR Gene]], [[Part:BBa K4226002|hrpS Gene]] and hrpL promoter.
  
  
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<partinfo>BBa_K4226003 parameters</partinfo>
 
<partinfo>BBa_K4226003 parameters</partinfo>
 
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<b>Reference</b>
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<br>
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[1] Wan, Xinyi, et al. "Cascaded amplifying circuits enable ultrasensitive cellular sensors for toxic metals." Nature chemical biology 15.5 (2019): 540-548.<br>

Latest revision as of 10:03, 11 October 2022


hrpL Promoter

This part is an improvement for the part：Promoter hrpL uploaded by iGEM13_HIT-Harbin. This promoter will be active, when both hrpR Gene and hrpS Gene are present. The hrpL Promoter is transcriptionally upregulated by hrpR and hrpS. The Amp30E Amplification Device consists of hrpR Gene, hrpS Gene and hrpL promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Reference
[1] Wan, Xinyi, et al. "Cascaded amplifying circuits enable ultrasensitive cellular sensors for toxic metals." Nature chemical biology 15.5 (2019): 540-548.