Difference between revisions of "Part:BBa K4226001"
 
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<partinfo>BBa_K4226001 short</partinfo>
 
<partinfo>BBa_K4226001 short</partinfo>
  
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This part is an improvement for the part：[[Part:BBa_K1014001|HrpR Gene]] uploaded by iGEM13_HIT-Harbin. The hrpR gene codes for hrpR protein. hrpR protein binds to hrpS protein forming a complex, and the promoter hrpL ([[Part:BBa K4226003|hrpL Promoter]]) is transcriptionally upregulated by hrpR and hrpS ([[Part:BBa K4226002|hrpS Gene]]) . The Amp30E Amplification Device consists of hrpR, hrpS ([[Part:BBa K4226002|hrpS Gene]]), and hrpL promoter ([[Part:BBa K4226003|hrpL Promoter]]) .
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This part is an improvement for the part：[[Part:BBa_K1014001|HrpR Gene]] uploaded by iGEM13_HIT-Harbin. The hrpR gene codes for hrpR protein. hrpR protein binds to hrpS protein forming a complex, and the [[Part:BBa K4226003|hrpL Promoter]] is transcriptionally upregulated by hrpR and [[Part:BBa K4226002|hrpS Gene]]. The [[Part:BBa_K4226004|Amp30E Amplification Device]] consists of hrpR, [[Part:BBa K4226002|hrpS Gene]] and [[Part:BBa K4226003|hrpL Promoter]].
  
  
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<b>Reference</b>
 
<b>Reference</b>
 
<br>
 
<br>
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[1]Wan, Xinyi, et al. "Cascaded amplifying circuits enable ultrasensitive cellular sensors for toxic metals." Nature chemical biology 15.5 (2019): 540-548.<br>
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[1] Wan, Xinyi, et al. "Cascaded amplifying circuits enable ultrasensitive cellular sensors for toxic metals." Nature chemical biology 15.5 (2019): 540-548.<br>

Latest revision as of 09:58, 11 October 2022


hrpR Gene

This part is an improvement for the part：HrpR Gene uploaded by iGEM13_HIT-Harbin. The hrpR gene codes for hrpR protein. hrpR protein binds to hrpS protein forming a complex, and the hrpL Promoter is transcriptionally upregulated by hrpR and hrpS Gene. The Amp30E Amplification Device consists of hrpR, hrpS Gene and hrpL Promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference
[1] Wan, Xinyi, et al. "Cascaded amplifying circuits enable ultrasensitive cellular sensors for toxic metals." Nature chemical biology 15.5 (2019): 540-548.