Difference between revisions of "Part:BBa K4361114"
| Line 3: | Line 3: | ||
<partinfo>BBa_K4361114 short</partinfo> | <partinfo>BBa_K4361114 short</partinfo> | ||
| − | + | This part shows the Blc operator sequence ([[Part:BBa_K4361111]]) after site-directed mutagenesis with primers [[Part:BBa_K4361112]] and [[Part:BBa_K4361113]], resulting in the deletion of the adenine in position 20. This mutation is expected to disrupt BlcR binding to the operator, meaning the protein cannot inhibit downstream gene expression. As such, when inserted in a reporter system such as [[Part:BBa_K4361115]], it may act as a negative control against the wildtype operator sequence. | |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 09:00, 11 October 2022
Blc operator sequence SDM deletion
This part shows the Blc operator sequence (Part:BBa_K4361111) after site-directed mutagenesis with primers Part:BBa_K4361112 and Part:BBa_K4361113, resulting in the deletion of the adenine in position 20. This mutation is expected to disrupt BlcR binding to the operator, meaning the protein cannot inhibit downstream gene expression. As such, when inserted in a reporter system such as Part:BBa_K4361115, it may act as a negative control against the wildtype operator sequence.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]