Difference between revisions of "Part:BBa K4165226"

Latest revision as of 08:49, 11 October 2022

Linker GGSGG

Flexible linker with 5 aa sequences.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Dry Lab Characterization

In our project, we generated a library for GS flexible linkers to be tested on different parts of our system; each linker was modeled in the fusion protein, and we filtered through them until we found the suitable length to use in linking our PROTAC, clamps, and switches together.

References

1- Yuan, S., Chan, H. S., & Hu, Z. (2017). Using pymol as a platform for computational drug design. WIREs Computational Molecular Science, 7(2). doi:10.1002/wcms.1298

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4165226 short</partinfo>
-flexible linker with 5 aa sequence
+Flexible linker with 5 aa sequences.
-===Usage and Biology===
-A flexible linker with G and S aa residues with variable lengths. According to our project, 3 flexible linker required one for our clamps for binding of Tau or amyloid beta with our binding paptides . Second one for flexible linking between clamps and HTRA1 binding peptide . last one for binding our HTRA1 binding peptide with PDZ domain of our protease HTRA1. our linker with length [5 aa GGSGGG ] was calculated by our team for testing how it would affect interaction of our mentioned parts with each other using Pymol Software.
@@ Line 13: / Line 9: @@
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K4165226 SequenceAndFeatures</partinfo>
+===Dry Lab Characterization===
+In our project, we generated a library for GS flexible linkers to be tested on different parts of our system; each linker was modeled in the fusion protein, and we filtered through them until we found the suitable length to use in linking our PROTAC, clamps, and switches together.
 ===References===