Difference between revisions of "Part:BBa K4361113"

Revision as of 08:47, 11 October 2022

Blc operator SDM primer R

A site-directed mutagenesis primer, to be used in conjunction with the mutation-inducing forward primer Part:BBa_K4361112. When used as primers during the PCR of pSB1C3 containing the Blc operator sequence as an insert (Part:BBa_K4361115), a deletion will be induced in the operator, resulting in the altered sequence as described in Part:BBa_K4361114. As this deletion occurs in the DNA sequence which is recognized and bound by BlcR, it is expected to disrupt binding of BlcR. Thus the resulting mutated operator sequence may act as a negative control when testing the binding properties of BlcR.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 21
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 21
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 21
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 21
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 21
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4361113 short</partinfo>
-A site-directed mutagenesis primer, to be used in conjunction with the mutation-inducing forward primer [[Part:BBa_K4361112]]. When used as primers during the PCR of pSB1C3 containing the Blc operator sequence as an insert ([[Part:BBa_K4361115]]), a deletion will be induced in the operator, resulting in the altered sequence as described in [[Part:BBa_K4361114]]. As this deletion occurs in the DNA sequence which is recognized and bound by BlcR, it is expected to disrupt binding of BlcR. Thus the resulting mutated operator sequence ([[Part:BBa_K4361114]]) may act as a negative control when testing the binding properties of BlcR.
+A site-directed mutagenesis primer, to be used in conjunction with the mutation-inducing forward primer [[Part:BBa_K4361112]]. When used as primers during the PCR of pSB1C3 containing the Blc operator sequence as an insert ([[Part:BBa_K4361115]]), a deletion will be induced in the operator, resulting in the altered sequence as described in [[Part:BBa_K4361114]]. As this deletion occurs in the DNA sequence which is recognized and bound by BlcR, it is expected to disrupt binding of BlcR. Thus the resulting mutated operator sequence may act as a negative control when testing the binding properties of BlcR.
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