Difference between revisions of "Part:BBa K4361112"
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A site-directed mutagenesis primer, to be used in conjunction with the reverse primer [[Part:BBa_K4361113]]. When used as primers during the PCR of pSB1C3 containing the Blc operator sequence as an insert ([[Part:BBa_K4361115]]), a deletion will be induced in the operator, resulting in the altered sequence as described in [[Part:BBa_K4361114]]. As this deletion occurs in the DNA sequence which is recognized and bound by BlcR, it is expected to disrupt binding of BlcR. Thus the resulting mutated operator sequence may act as a negative control when testing the binding properties of BlcR.
 
A site-directed mutagenesis primer, to be used in conjunction with the reverse primer [[Part:BBa_K4361113]]. When used as primers during the PCR of pSB1C3 containing the Blc operator sequence as an insert ([[Part:BBa_K4361115]]), a deletion will be induced in the operator, resulting in the altered sequence as described in [[Part:BBa_K4361114]]. As this deletion occurs in the DNA sequence which is recognized and bound by BlcR, it is expected to disrupt binding of BlcR. Thus the resulting mutated operator sequence may act as a negative control when testing the binding properties of BlcR.
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A site-directed mutagenesis primer, used to induce the deletion of adenine 20 in the Blc operator sequence ([[Part:BBa_K4361111]]) during PCR, in conjunction with the reverse primer [[Part:BBa_K4361113]]. As this deletion occurs in the DNA sequence which is recognized and bound by BlcR, it is expected to disrupt binding of BlcR. Thus the resulting mutated operator sequence ([[Part:BBa_K4361114]]) may act as a negative control when testing the binding properties of BlcR.
 
  
 
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Revision as of 08:46, 11 October 2022


Blc operator SDM primer F

A site-directed mutagenesis primer, to be used in conjunction with the reverse primer Part:BBa_K4361113. When used as primers during the PCR of pSB1C3 containing the Blc operator sequence as an insert (Part:BBa_K4361115), a deletion will be induced in the operator, resulting in the altered sequence as described in Part:BBa_K4361114. As this deletion occurs in the DNA sequence which is recognized and bound by BlcR, it is expected to disrupt binding of BlcR. Thus the resulting mutated operator sequence may act as a negative control when testing the binding properties of BlcR.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]