Difference between revisions of "Part:BBa K4361113"

 
Line 3: Line 3:
 
<partinfo>BBa_K4361113 short</partinfo>
 
<partinfo>BBa_K4361113 short</partinfo>
  
−
TBD
+
A site-directed mutagenesis primer, to be used in conjunction with the mutation-inducing forward primer [[Part:BBa_K4361112]]. When used as primers during the PCR of pSB1C3 containing the Blc operator sequence as an insert ([[Part:BBa_K4361115]]), a deletion will be induced in the operator, resulting in the altered sequence as described in [[Part:BBa_K4361114]]. As this deletion occurs in the DNA sequence which is recognized and bound by BlcR, it is expected to disrupt binding of BlcR. Thus the resulting mutated operator sequence ([[Part:BBa_K4361114]]) may act as a negative control when testing the binding properties of BlcR.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 9: Line 9:
  
 
<!-- -->
 
<!-- -->
−
<span class='h3bb'>Sequence and Features</span>
+
<span class='h3bb'><h3>Sequence and Features</h3></span>
 
<partinfo>BBa_K4361113 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4361113 SequenceAndFeatures</partinfo>
  

Revision as of 08:45, 11 October 2022


Blc operator SDM primer R

A site-directed mutagenesis primer, to be used in conjunction with the mutation-inducing forward primer Part:BBa_K4361112. When used as primers during the PCR of pSB1C3 containing the Blc operator sequence as an insert (Part:BBa_K4361115), a deletion will be induced in the operator, resulting in the altered sequence as described in Part:BBa_K4361114. As this deletion occurs in the DNA sequence which is recognized and bound by BlcR, it is expected to disrupt binding of BlcR. Thus the resulting mutated operator sequence (Part:BBa_K4361114) may act as a negative control when testing the binding properties of BlcR.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 21
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 21
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 21
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 21
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 21
  • 1000
    COMPATIBLE WITH RFC[1000]