Difference between revisions of "Part:BBa K4361112"
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Revision as of 08:41, 11 October 2022
Blc operator SDM primer F
A site-directed mutagenesis primer, used to induce the deletion of adenine 20 in the Blc operator sequence (Part:BBa_K4361111) during PCR, in conjunction with the reverse primer Part:BBa_K4361113. As this deletion occurs in the DNA sequence which is recognized and bound by BlcR, it is expected to disrupt binding of BlcR. Thus the resulting mutated operator sequence (Part:BBa_K4361114) may act as a negative control when testing the binding properties of BlcR.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]