Difference between revisions of "Part:BBa K4361112"

 
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<partinfo>BBa_K4361112 short</partinfo>
 
<partinfo>BBa_K4361112 short</partinfo>
  
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TBD
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A site-directed mutagenesis primer, used to induce the deletion of adenine 20 in the Blc operator sequence ([[Part:BBa_K4361111]]) during PCR, in conjunction with the reverse primer [[Part:BBa_K4361113]]. As this deletion occurs in the DNA sequence which is recognized and bound by BlcR, it is expected to disrupt binding of BlcR. Thus the resulting mutated operator sequence ([[Part:BBa_K4361114]]) may act as a negative control when testing the binding properties of BlcR.
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Largely overlaps with BBa_K4361111, but with a deletion of the adenine in position 20 to disrupt BlcR binding.
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Revision as of 08:41, 11 October 2022


Blc operator SDM primer F

A site-directed mutagenesis primer, used to induce the deletion of adenine 20 in the Blc operator sequence (Part:BBa_K4361111) during PCR, in conjunction with the reverse primer Part:BBa_K4361113. As this deletion occurs in the DNA sequence which is recognized and bound by BlcR, it is expected to disrupt binding of BlcR. Thus the resulting mutated operator sequence (Part:BBa_K4361114) may act as a negative control when testing the binding properties of BlcR.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]