Difference between revisions of "Part:BBa K4139000"

Latest revision as of 08:29, 11 October 2022

mcyH ABC Transporter for Microcystin Production

The mcyH gene is a member of the mcy gene cluster in Microcystis aeruginosa cyanobacteria. The mcy gene cluster is responsible for the production of microcystins, which are hepatotoxins produced by the bacteria. The mcyH gene encodes an ATP binding cassette (ABC) transporter, which is essential for microcystin production. This part was designed so that the mcyH gene could be expressed in E.coli; mcyH is the target sequence for a Cas13a crRNA designed to target microcystis aeruginosa, so by designing a version of this part to be expressed in E.coli, we can test the target specificity of our Cas13a and crRNA in the lab without needing to work with toxic cyanobacteria.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 744
Illegal AgeI site found at 68
Illegal AgeI site found at 1132
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K4139000 short</partinfo>
-The mcyH gene is a member of the mcy gene cluster in Microcystis aeruginosa cyanobacteria. The mcy gene cluster is responsible for the production of microcystins, which are hepatotoxins produced by the bacteria. The mcyH gene encodes an ATP binding cassette (ABC) transporter, which is essential for microcystin production.
+The mcyH gene is a member of the mcy gene cluster in Microcystis aeruginosa cyanobacteria. The mcy gene cluster is responsible for the production of microcystins, which are hepatotoxins produced by the bacteria. The mcyH gene encodes an ATP binding cassette (ABC) transporter, which is essential for microcystin production. This part was designed so that the mcyH gene could be expressed in E.coli; mcyH is the target sequence for a Cas13a crRNA designed to target microcystis aeruginosa, so by designing a version of this part to be expressed in E.coli, we can test the target specificity of our Cas13a and crRNA in the lab without needing to work with toxic cyanobacteria.
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