Difference between revisions of "Part:BBa K4365021:Design"
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===Source=== | ===Source=== | ||
− | + | Construct was ordered from IDT as gBlock, cloned into the p426 plasmid and fused to the N-terminus of the turboRFP fluorescent protein. | |
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===References=== | ===References=== |
Revision as of 08:25, 11 October 2022
SP-SUMO-turboRFP
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 1001
Illegal PstI site found at 1036
Illegal PstI site found at 1335 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 1001
Illegal PstI site found at 1036
Illegal PstI site found at 1335 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2168
Illegal BamHI site found at 1007 - 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 1001
Illegal PstI site found at 1036
Illegal PstI site found at 1335 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 1001
Illegal PstI site found at 1036
Illegal PstI site found at 1335
Illegal NgoMIV site found at 172 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
yeast
Source
Construct was ordered from IDT as gBlock, cloned into the p426 plasmid and fused to the N-terminus of the turboRFP fluorescent protein.