Difference between revisions of "Part:BBa K4156081"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | In order to obtain the highest level of translation from this RBS, it must be linked to a coding region preceded by a coding region. A standard prefix increases the distance between the RBS and the start codon and decreases the translation efficiency. In our experiments, we use hyper-spank in combination with spoVG and use this to form the pLldR subunit (<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_BBa_K4156101"> | + | In order to obtain the highest level of translation from this RBS, it must be linked to a coding region preceded by a coding region. A standard prefix increases the distance between the RBS and the start codon and decreases the translation efficiency. In our experiments, we use hyper-spank in combination with spoVG and use this to form the pLldR subunit (<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_BBa_K4156101"> BBa_K4156101 </a></html>) |
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Latest revision as of 08:25, 11 October 2022
RBS spoVG
SpoVG is an endogenous ribosome binding site (RBS).
Usage and Biology
In order to obtain the highest level of translation from this RBS, it must be linked to a coding region preceded by a coding region. A standard prefix increases the distance between the RBS and the start codon and decreases the translation efficiency. In our experiments, we use hyper-spank in combination with spoVG and use this to form the pLldR subunit ( BBa_K4156101 )
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]