Difference between revisions of "Part:BBa K4165063"
 
(2 intermediate revisions by 2 users not shown)
Line 4: Line 4:
  
 
A flexible 7 aa Glycine-Serine linker
 
A flexible 7 aa Glycine-Serine linker
−
 
−
===Usage and Biology===
 
−
A flexible linker with G and S aa residues with variable lengths. According to our project, 3 flexible linker required one for our clamps for binding of Tau or amyloid beta with our binding peptides. Second one for flexible linking between clamps and HTRA1 binding peptide. last one for binding our HTRA1 binding peptide with PDZ domain of our protease HTRA1. our linker with length [7 aa GSGSGSG ] was calculated by our team for testing how it would affect interaction of our mentioned parts with each other using Pymol Software.
 
  
 
<!-- -->
 
<!-- -->
−
<span class='h3bb'>Sequence and Features</span>
+
===<span class='h3bb'>Sequence and Features</span>===
 
<partinfo>BBa_K4165063 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4165063 SequenceAndFeatures</partinfo>
  
 +
 +
===Dry Lab Characterization===
 +
In our project, we generated a library for GS flexible linkers to be tested on different parts of our system; each linker was modeled in the fusion protein, and we filtered through them until we found the suitable length to use in linking our PROTAC, clamps, and switches together.
 +
 +
===References===
 +
1- Yuan, S., Chan, H. S., & Hu, Z. (2017). Using pymol as a platform for computational drug design. WIREs Computational Molecular Science, 7(2). doi:10.1002/wcms.1298
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 08:25, 11 October 2022


GSGSGSG

A flexible 7 aa Glycine-Serine linker

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Dry Lab Characterization

In our project, we generated a library for GS flexible linkers to be tested on different parts of our system; each linker was modeled in the fusion protein, and we filtered through them until we found the suitable length to use in linking our PROTAC, clamps, and switches together.

References

1- Yuan, S., Chan, H. S., & Hu, Z. (2017). Using pymol as a platform for computational drug design. WIREs Computational Molecular Science, 7(2). doi:10.1002/wcms.1298