Difference between revisions of "Part:BBa K4164013"
Line 3: Line 3:
 
<partinfo>BBa_K4164013 short</partinfo>
 
<partinfo>BBa_K4164013 short</partinfo>
  
−
In order to find an appropriate expression intensity to achieve balance between metabolic burden and detection efficiency, we also tried T7lac promoter from pET-29a+(Novagen).
+
In order to find an appropriate expression intensity to achieve balance between metabolic burden and detection efficiency, we also tried T7<em>lac</em> promoter from pET-29a+(Novagen).
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 08:24, 11 October 2022


Inductive expression of BSS

In order to find an appropriate expression intensity to achieve balance between metabolic burden and detection efficiency, we also tried T7lac promoter from pET-29a+(Novagen).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1600
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1001
    Illegal NgoMIV site found at 1052
  • 1000
    COMPATIBLE WITH RFC[1000]