Difference between revisions of "Part:BBa K4365021"

 
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<partinfo>BBa_K4365021 short</partinfo>
 
<partinfo>BBa_K4365021 short</partinfo>
  
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BBa_K849009 terminator
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SP-SUMO-tRFP is a reporter device which could be used to test protein secretion in yeast. Moreover if tRFP is exchanged for the protein of interest after protein purification with His-tag you scarlessly remove the tag from the protein with a SUMO protease. This part is optimized for expression in <i>Saccharomyces cerevisiae</i>.
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BBa_K3033006 his-tag
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fig1, alpha, glyc linker,his-tag, sumo, trfp, terminator
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SP-SUMO-tRFP consists of:
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BBa_K4365000, BBa_K4365019, BBa_K4365005, BBa_K3033006, BBa_K4365004, BBa_K4365001, BBa_K849009
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* FIG1 promoter [https://parts.igem.org/Part:BBa_K4365000 K4365000]
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* signal peptide (alpha mating phactor signal peptide) [https://parts.igem.org/Part:BBa_K4365019 K4365019]
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* glycine linker [https://parts.igem.org/Part:BBa_K4365005 K4365005]
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* 6x His-Tag [https://parts.igem.org/Part:BBa_K3033006 K3033006]
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* SUMO [https://parts.igem.org/Part:BBa_K4365004 K4365004]
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* codon optimized turboRFP [https://parts.igem.org/Part:BBa_K4365020 K4365020]
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* terminator tCYC1 [https://parts.igem.org/Part:BBa_K849009 K849009]
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 +
<br><span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4365021 SequenceAndFeatures</partinfo>
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 +
__TOC__
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
  
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<!-- -->
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4365021 SequenceAndFeatures</partinfo>
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Revision as of 08:16, 11 October 2022


SP-SUMO-turboRFP

SP-SUMO-tRFP is a reporter device which could be used to test protein secretion in yeast. Moreover if tRFP is exchanged for the protein of interest after protein purification with His-tag you scarlessly remove the tag from the protein with a SUMO protease. This part is optimized for expression in Saccharomyces cerevisiae.

SP-SUMO-tRFP consists of:


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1001
    Illegal PstI site found at 1036
    Illegal PstI site found at 1335
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1001
    Illegal PstI site found at 1036
    Illegal PstI site found at 1335
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2168
    Illegal BamHI site found at 1007
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1001
    Illegal PstI site found at 1036
    Illegal PstI site found at 1335
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1001
    Illegal PstI site found at 1036
    Illegal PstI site found at 1335
    Illegal NgoMIV site found at 172
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology