Difference between revisions of "Part:BBa K4164003"

Latest revision as of 06:51, 11 October 2022

Retinoid X receptor-alpha(RXRα)

Retinoid X receptor alpha (RXR-alpha), also known as NR2B1 (nuclear receptor subfamily 2, group B, member 1) is a nuclear receptor that in humans is encoded by the RXRA gene.

RXR is associated with multiple cellular processes from cell proliferation to cell metabolism. RXRs have the ability to form heterodimers with subfamily 1 nuclear receptors including CAR, FXR, LXR, PPAR， PXR， RAR， TR and VDR.

As with other type II nuclear receptors, the RXR heterodimer in the absence of ligand is bound to hormone response elements complexed with corepressor proteins. Binding of agonist ligands to RXR results in dissociation of corepressor and recruitment of coactivator protein, which, in turn, promotes transcription of the downstream target gene into mRNA and eventually protein. Alternatively, binding of RXR ligands to heterodimers can stimulate transcriptional activation of RXR chaperone receptors. For example, when binding as a heterodimer with activated FXR in liver cells, it will regulate the metabolic processes of bile acid (BA), lipid and glucose.

In our project, we took advantage of the characteristics of RXR that it can bind to the farnesoid X receptor(FXR) as a heterodimer when activated by de-sulfated CDCA-S. After it forms a heterodimeric complex with FXR, the linked ddRFP-A1 and ddRFP-B1 are correspondingly driven to dimerize and emit fluorescence.

Sequence and Features