Difference between revisions of "Part:BBa K4146505"

Revision as of 06:26, 11 October 2022

pet-lacI-lac-aro10-adh6-T1

This composite element mainly contains two structural sequences. One is aro10, which is used to encode and synthesize Phynpyruvate decarboxylase. The other is adh6, which can help forming 2-phenylethanol, from phenylacetaldehyde.

Fig.1 The standard curve for determination of 2-phenylethanol yield.

In the standard curve, as shown in Fig.1,the ordinate is the peak area and the abscissa is the marker concentration.The mobile phase of high performance liquid chromatography is trifluoroacetic acid diluted 1000 times.In our experiment, we took two points, 500 mg/L and 1000 mg/L, as well as zero point. The R square fitted by the standard curve is 1, which shows that the experimental results are fitted very well, and the error can be almost ignored, that is, the measured output is relatively accurate.

Fig.2 The experimental 2-phenylethanol yield.

According to the data read out on the standard curve, we have drawn the curve of 2-PE concentration versus time ,as shown in Fig.2.

Sequence and Features