Difference between revisions of "Part:BBa K4317001"

Latest revision as of 05:46, 11 October 2022

P2069M

P2069 originated from B. pumilus BA06. P2069M, engineered by Cui-Cui Miao and colleagues, has strong promoter activity in Bacillus subtilis.

Usage and Biology

Figure 1. Expression of mScarlet by various promoter in Bacillus subtilis 1A976 and Escherichia coli BL21(de3)

References

Miao, Cui-Cui, et al. "Construction of a high-expression system in Bacillus through transcriptomic profiling and promoter engineering." Microorganisms 8.7 (2020): 1030.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 7
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 7
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 7
Illegal BglII site found at 13
Illegal BamHI site found at 1
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 7
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 7
1000
COMPATIBLE WITH RFC[1000]

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-__NOTOC__
 <partinfo>BBa_K4317001 short</partinfo>
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 https://static.igem.org/mediawiki/parts/c/cb/P2069M_ref.png
-Microorganisms 2020, 8, 1030; doi:10.3390/microorganisms8071030
 https://static.igem.org/mediawiki/parts/thumb/2/24/P2069M.png/800px-P2069M.png
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
 https://static.igem.org/mediawiki/parts/thumb/5/51/Promoter_strength.png/800px-Promoter_strength.png
-<!-- -->
+Figure 1. Expression of mScarlet by various promoter in Bacillus subtilis 1A976 and Escherichia coli BL21(de3)
+===References===
+Miao, Cui-Cui, et al. "Construction of a high-expression system in Bacillus through transcriptomic profiling and promoter engineering." Microorganisms 8.7 (2020): 1030.
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K4317001 SequenceAndFeatures</partinfo>