Difference between revisions of "Part:BBa K176180:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K176180 short</partinfo>
 
<partinfo>BBa_K176180 short</partinfo>
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pCon 0.15(K176008) generates TetR-LVA
 
pCon 0.15(K176008) generates TetR-LVA
  
−
pCon 0.04(J23103) generates LuxR
+
pCon 0.04(J23109) generates LuxR
  
 
pLux/Tet(K176000) generates GFP, activated by LuxR and Input2 AHL, repressed by TetR-LVA and no Input1 aTc
 
pLux/Tet(K176000) generates GFP, activated by LuxR and Input2 AHL, repressed by TetR-LVA and no Input1 aTc

Latest revision as of 14:06, 7 October 2009

aTc&AHL->GFP: pCon 0.15->tetR-LVA+pCon 0.04->luxR+pLux/Tet->GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 898
    Illegal NheI site found at 921
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1876
    Illegal BsaI.rc site found at 2620


Design Notes

Input1: aTc

Input2: AHL

Output: GFP

Design:

pCon 0.15(K176008) generates TetR-LVA

pCon 0.04(J23109) generates LuxR

pLux/Tet(K176000) generates GFP, activated by LuxR and Input2 AHL, repressed by TetR-LVA and no Input1 aTc


Source

K176008 K176174

References