Difference between revisions of "Part:BBa K4182007:Design"

(Design Notes)
(Profile)
Line 7: Line 7:
  
 
==Profile==
 
==Profile==
 +
===Base Pairs===
 +
273
 +
 
===Design Notes===
 
===Design Notes===
 
The gene was optimized by E. coli codon
 
The gene was optimized by E. coli codon
  
 
===Source===
 
===Source===
 
 
Shigella flexneri 2a str. 301 (strain: 301, serotype: 2a)
 
Shigella flexneri 2a str. 301 (strain: 301, serotype: 2a)
  
===References===
+
==Usage&Biology==
 +
 
 +
==References==
 +
 
 +
1. Din, M.O., et al., Synchronized cycles of bacterial lysis for in vivo delivery. Nature, 2016. 536(7614): p. 81-85.
 +
 
 +
2. Saeidi, N., et al., Engineering microbes to sense and eradicate Pseudomonas aeruginosa, a human pathogen. Mol Syst Biol, 2011. 7: p. 521.
 +
 
 +
3. Restrepo-Pineda, S., et al., Thermoinducible expression system for producing recombinant proteins in Escherichia coli: advances and insights. FEMS Microbiol Rev, 2021. 45(6).
 +
 
 +
4. Aparicio, T., V. de Lorenzo, and E. Martínez-García, Improved Thermotolerance of Genome-Reduced Pseudomonas putida EM42 Enables Effective Functioning of the PL/cI857 System. Biotechnology Journal, 2019. 14(1): p. 1800483.

Revision as of 03:55, 11 October 2022


Lysis-phi X174


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Profile

Base Pairs

273

Design Notes

The gene was optimized by E. coli codon

Source

Shigella flexneri 2a str. 301 (strain: 301, serotype: 2a)

Usage&Biology

References

1. Din, M.O., et al., Synchronized cycles of bacterial lysis for in vivo delivery. Nature, 2016. 536(7614): p. 81-85.

2. Saeidi, N., et al., Engineering microbes to sense and eradicate Pseudomonas aeruginosa, a human pathogen. Mol Syst Biol, 2011. 7: p. 521.

3. Restrepo-Pineda, S., et al., Thermoinducible expression system for producing recombinant proteins in Escherichia coli: advances and insights. FEMS Microbiol Rev, 2021. 45(6).

4. Aparicio, T., V. de Lorenzo, and E. Martínez-García, Improved Thermotolerance of Genome-Reduced Pseudomonas putida EM42 Enables Effective Functioning of the PL/cI857 System. Biotechnology Journal, 2019. 14(1): p. 1800483.