Difference between revisions of "Part:BBa K4245009"

Revision as of 02:21, 11 October 2022

hsa-mir-133a-3p

This part is the sequence for hsa-miR-133a-3p, a miRNA isolated from Homo sapiens. This miRNA acts as an upregulated biomarker for coronary artery disease (Kaur et al., 2020), and is therefore potentially useful for the early detection of this condition. Similar to BBa_K4245006, hsa-miR-133a-3p, or miRNA-133a-3p, is seen to increase in blood circulation potentially hours and days before a myocardial infarction, even before Troponin T is increased in circulation, and it is also related with cardiac-specific muscle lineage (Xiao et al., 2019). Thus, both biomarkers increase the sensitivity of our biosensors, as both miRNA-1-3p and miRNA-133a-3p in higher concentrations could indicate a possible heart attack.

The Lambert_GA 2022 team developed a set of padlock probes to use the rolling circle amplification approaches for several miRNAs related to CAD. This miRNA is used as the basis for BBa_K4245103 and BBa_K4245110, the 3' arm for hsa-miR-133a-3p and 5' arm for hsa-miR-133a-3p, and as the target sequence for BBa_K4245204 and BBa_K4245205 the hsa-miR-133a-3p RCA Padlock Probe and the hsa-miR-133a-3p RCT Padlock Probe.

When using rolling circle amplification (RCA), the miRNA binds to the padlock. A rolling circle product (RCP) is produced from BBa_K4245131 (Middle Sequence), which is then detected by the linear probes BBa_K4245130 (Fluorophore) and BBa_K4245132 (Quencher). When these parts bind to the RCP, the fluorescence decreases. Therefore, lower fluorescence is indicative of greater miRNA concentrations.

References:

Kaur, A., Mackin, S. T., Schlosser, K., Wong, F. L., Elharram, M., Delles, C., Stewart, D. J., Dayan, N., Landry, T., & Pilote, L. (2020). Systematic review of microRNA biomarkers in acute coronary syndrome and stable coronary artery disease. Cardiovascular research, 116(6), 1113–1124. https://doi.org/10.1093/cvr/cvz302
Xiao, Y., Zhao, J., Tuazon, J. P., Borlongan, C. V., & Yu, G. (2019). MicroRNA-133a and Myocardial Infarction. Cell transplantation, 28(7), 831–838. https://doi.org/10.1177/0963689719843806

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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-This part is the sequence for hsa-miR-133a-3p, an miRNA isolated from <i> Homo sapiens </i>. This miRNA acts as an upregulated biomarker for coronary artery disease (Kaur et al., 2020), and is therefore potentially useful for the early detection of this condition. Similar to <partinfo>BBa_K4245006</partinfo>, hsa-miR-133a-3p, or miRNA-133a-3p, is seen to increase in blood circulation potentially hours and days before a myocardial infarction, even before Troponin T is increased in circulation, and it is also related with cardiac-specific muscle lineage (Xiao et al., 2019). Thus, both biomarkers increase the sensitivity of our biosensors, as both miRNA-1-3p and miRNA-133a-3p in higher concentrations could indicate a possible heart attack.
+This part is the sequence for hsa-miR-133a-3p, a miRNA isolated from <i>Homo sapiens</i>. This miRNA acts as an upregulated biomarker for coronary artery disease (Kaur et al., 2020), and is therefore potentially useful for the early detection of this condition. Similar to <partinfo>BBa_K4245006</partinfo>, hsa-miR-133a-3p, or miRNA-133a-3p, is seen to increase in blood circulation potentially hours and days before a myocardial infarction, even before Troponin T is increased in circulation, and it is also related with cardiac-specific muscle lineage (Xiao et al., 2019). Thus, both biomarkers increase the sensitivity of our biosensors, as both miRNA-1-3p and miRNA-133a-3p in higher concentrations could indicate a possible heart attack.
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