Difference between revisions of "Part:BBa K3748015"
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pREV1 promoters showed a constitutive level of Venus fluorescence throughout the experiment, confirming that it is a constitutive promoter. Compared to the background fluorescence of the DOM90 strain pREV1 demonstrated a five-fold higher level of fluorescence intensity (Fig. 1). | pREV1 promoters showed a constitutive level of Venus fluorescence throughout the experiment, confirming that it is a constitutive promoter. Compared to the background fluorescence of the DOM90 strain pREV1 demonstrated a five-fold higher level of fluorescence intensity (Fig. 1). | ||
| − | [[Image:PREV1 Estonia TUIT | + | [[Image:PREV1 Estonia TUIT new.png|600px|thumb|center|<b>Figure 3. The expression level of Venus is controlled by pREV1</b>. Bars indicate the mean fluorescence signal (AU), and error bars show the standard deviation. ]] |
<b>References:</b> | <b>References:</b> | ||
Revision as of 02:18, 11 October 2022
pREV1
Weak strenght yeast promoter S.cerevisiae.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 705
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Team Estonia_TUIT characterization of BBa_K3748015 (pREV1)
pREV1 is a constitutive promoter responsible for the expression of REV1, a gene encoding bi-functional DNA-directed DNA polymerase/deoxycytidyl transferase (Lee et al., 2015). This enzyme is involved in error-prone translesion synthesis, one of the pathways for DNA repair (Lawrence, 2004).
pREV1 promoters showed a constitutive level of Venus fluorescence throughout the experiment, confirming that it is a constitutive promoter. Compared to the background fluorescence of the DOM90 strain pREV1 demonstrated a five-fold higher level of fluorescence intensity (Fig. 1).
References:
- Lee, M. E., DeLoache, W. C., Cervantes, B., & Dueber, J. E. (2015). A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. ACS Synthetic Biology, 4(9), 975–986. https://doi.org/10.1021/SB500366V/SUPPL_FILE/SB500366V_SI_002.ZIP
- Lawrence, C. W. (2004). Cellular functions of DNA polymerase ζ and REV1 protein. Advances in Protein Chemistry, 69, 167–203. https://doi.org/10.1016/S0065-3233(04)69006-1