Difference between revisions of "Part:BBa K3748015"

(→‎Team Estonia_TUIT characterization of BBa_K3748015 (pREV1))
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<partinfo>BBa_K3748015 short</partinfo>
 
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==Team Estonia_TUIT characterization of BBa_K3748015 (<i>pREV1</i>)==
 
==Team Estonia_TUIT characterization of BBa_K3748015 (<i>pREV1</i>)==
  
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<i>pREV1</i> is a constitutive promoter (Michael E. Lee et. al, 2015) responsible for the expression of <i>Rev1</i>, a gene encoding bi-functional DNA-directed DNA polymerase/deoxycytidyl transferase. This enzyme is involved in error-prone translesion synthesis, one of the pathways for DNA repair (Lawrence CW, 2004).
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pREV1 is a constitutive promoter responsible for the expression of REV1, a gene encoding bi-functional DNA-directed DNA polymerase/deoxycytidyl transferase (Lee <i>et al</i>., 2015). This enzyme is involved in error-prone translesion synthesis, one of the pathways for DNA repair (Lawrence, 2004).
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<i>pREV1</i> promoter showed constitutive levels of Venus fluorescence throughout the experiment. Compared to the background fluorescence of the DOM90 strain, <i>pREV1</i> demonstrated a five-fold increase in fluorescence intensity (figure 1).
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pREV1 promoters showed a constitutive level of Venus fluorescence throughout the experiment, confirming that it is a constitutive promoter. Compared to the background fluorescence of the DOM90 strain pREV1 demonstrated a five-fold higher level of fluorescence intensity (Fig. 1).
  
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[[Image:PREV1 Estonia TUIT.png.png|600px|thumb|center|'''Figure 1:''' <b>Graph depicting median fluorescence intensity of <i>pREV1</i> and control strain</b>]]
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[[Image:PREV1 Estonia TUIT.png.png|600px|thumb|center|<b>Figure 3. The expression level of Venus is controlled by pREV1</b>. Bars indicate the mean fluorescence signal (AU), and error bars show the standard deviation. ]]
  
 
<b>References:</b>
 
<b>References:</b>
 
<ul>
 
<ul>
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<li>A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. Michael E. Lee, William C. DeLoach, Bernardo Cervantes, and John E. Dueber, 2015</li>   
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<li>Lee, M. E., DeLoache, W. C., Cervantes, B., & Dueber, J. E. (2015). A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. <i>ACS Synthetic Biology, 4</i>(9), 975–986. https://doi.org/10.1021/SB500366V/SUPPL_FILE/SB500366V_SI_002.ZIP</li>   
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<li>Cellular functions of DNA polymerase zeta and Rev1 protein, Lawrence CW (2004), Adv Protein Chem</li>
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<li>Lawrence, C. W. (2004). Cellular functions of DNA polymerase ζ and REV1 protein. <i>Advances in Protein Chemistry, 69, </i>167–203. https://doi.org/10.1016/S0065-3233(04)69006-1</li>
 
</ul>
 
</ul>

Revision as of 02:16, 11 October 2022

pREV1

Weak strenght yeast promoter S.cerevisiae.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 705
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Team Estonia_TUIT characterization of BBa_K3748015 (pREV1)

pREV1 is a constitutive promoter responsible for the expression of REV1, a gene encoding bi-functional DNA-directed DNA polymerase/deoxycytidyl transferase (Lee et al., 2015). This enzyme is involved in error-prone translesion synthesis, one of the pathways for DNA repair (Lawrence, 2004).

pREV1 promoters showed a constitutive level of Venus fluorescence throughout the experiment, confirming that it is a constitutive promoter. Compared to the background fluorescence of the DOM90 strain pREV1 demonstrated a five-fold higher level of fluorescence intensity (Fig. 1).

Figure 3. The expression level of Venus is controlled by pREV1. Bars indicate the mean fluorescence signal (AU), and error bars show the standard deviation.

References: