Difference between revisions of "Part:BBa K4169016:Design"

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It comes from https://www.ebi.ac.uk/ena/browser/view/X89575 Coding: CDX46666.1.
 
It comes from https://www.ebi.ac.uk/ena/browser/view/X89575 Coding: CDX46666.1.
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===References===
 
===References===
 +
Yang CC, Packman LC, Scrutton NS. The primary structure of Hyphomicrobium X dimethylamine dehydrogenase. Relationship to trimethylamine dehydrogenase and implications for substrate recognition. Eur J Biochem. 1995 Aug 15;232(1):264-71. doi: 10.1111/j.1432-1033.1995.tb20808.x. PMID: 7556160.

Revision as of 01:39, 11 October 2022


produce DMADH


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 897
    Illegal AgeI site found at 1291
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

GenScript synthesised tmd plasmids and performed codon optimization.

We added His tag on the end of tmd sequence.

Source

It comes from https://www.ebi.ac.uk/ena/browser/view/X89575 Coding: CDX46666.1.


References

Yang CC, Packman LC, Scrutton NS. The primary structure of Hyphomicrobium X dimethylamine dehydrogenase. Relationship to trimethylamine dehydrogenase and implications for substrate recognition. Eur J Biochem. 1995 Aug 15;232(1):264-71. doi: 10.1111/j.1432-1033.1995.tb20808.x. PMID: 7556160.