Difference between revisions of "Part:BBa K4245009"

Revision as of 23:59, 10 October 2022

hsa-mir-133a-3p

This part is the sequence for hsa-miR-133a-3p, an miRNA isolated from Homo sapiens . This miRNA acts as an upregulated biomarker for coronary artery disease (Kaur et al., 2020), and is therefore potentially useful for the early detection of this condition. Similar to BBa_K4245006, hsa-miR-133a-3p, or miRNA-133a-3p, is seen to increase in blood circulation potentially hours and days before a myocardial infarction, even before Troponin T is increased in circulation, as it is also related with cardiac-specific muscle lineage (Xiao et al., 2019). Thus, both biomarkers increase the sensitivity of our biosensors, as both miRNA-1-3p and miRNA-133a-3p in higher concentration could indicate a possible heart attack.
The Lambert_GA 2022 team developed a set of padlock probes to be used with the rolling circle amplification approaches for several miRNAs related to CAD. This miRNA is used as the basis for BBa_K4245103 and BBa_K4245110, the 3' arm for hsa-miR-133a-3p and 5' arm for hsa-miR-133a-3p, and as the target sequence for BBa_K4245204 and BBa_K4245205 the hsa-miR-133a-3p RCA Padlock Probe and the hsa-miR-133a-3p RCT Padlock Probe. .
When using rolling circle amplification (RCA), the miRNA binds to the padlock. A rolling circle product (RCP) is produced from BBa_K4245131 (Middle Sequence), which is then detected by the linear probes BBa_K4245130 (Fluorophore) and BBa_K4245132 (Quencher). When these parts bind to the RCP, the fluorescence decreases. Therefore, lower fluorescence is indicative of greater miRNA concentrations.

References:

Kaur, A., Mackin, S. T., Schlosser, K., Wong, F. L., Elharram, M., Delles, C., Stewart, D. J., Dayan, N., Landry, T., & Pilote, L. (2020). Systematic review of microRNA biomarkers in acute coronary syndrome and stable coronary artery disease. Cardiovascular research, 116(6), 1113–1124. https://doi.org/10.1093/cvr/cvz302
Xiao, Y., Zhao, J., Tuazon, J. P., Borlongan, C. V., & Yu, G. (2019). MicroRNA-133a and Myocardial Infarction. Cell transplantation, 28(7), 831–838. https://doi.org/10.1177/0963689719843806

Sequence and Features