Difference between revisions of "Part:BBa J428351:Experience"
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===Experimental approach=== | ===Experimental approach=== | ||
=====JUMP DV Considerations===== | =====JUMP DV Considerations===== | ||
| − | Out of the 3 JUMP DVs that we characterised in <em>E. coli</em>, we aim to focus on pSC101 as its growth, compared to the other 2 DVs, was less sporadic and didn't go through such a dramatic death phase. Low copy number plasmids are often used as default as they exert less burden on cells so this allows us to made a larger contribution to the iGEM and synthetic biologist community. pSC101 being a low-copy number plasmid, will therefore exert less burden on our cells - this is in particular important for the construction and functionality of the complex 'robust perfect adaptation' circuit. | + | Out of the 3 JUMP DVs that we characterised in <em>E. coli</em>, we aim to focus on pSC101 as its growth, compared to the other 2 DVs, was less sporadic and didn't go through such a dramatic death phase, instead remaining at the stationary phase until the end of our experiment. Low copy number plasmids are also often used as a default as they exert less burden on cells so this allows us to made a larger contribution to the iGEM and synthetic biologist community. pSC101 being a low-copy number plasmid, will therefore exert less burden on our cells - this is in particular important for the construction and functionality of the complex 'robust perfect adaptation' circuit. |
===User Reviews=== | ===User Reviews=== | ||
Revision as of 22:56, 10 October 2022
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Applications of BBa_J428351
Cambridge 2022 JUMP DV Characterisation
Experimental approach
JUMP DV Considerations
Out of the 3 JUMP DVs that we characterised in E. coli, we aim to focus on pSC101 as its growth, compared to the other 2 DVs, was less sporadic and didn't go through such a dramatic death phase, instead remaining at the stationary phase until the end of our experiment. Low copy number plasmids are also often used as a default as they exert less burden on cells so this allows us to made a larger contribution to the iGEM and synthetic biologist community. pSC101 being a low-copy number plasmid, will therefore exert less burden on our cells - this is in particular important for the construction and functionality of the complex 'robust perfect adaptation' circuit.
User Reviews
UNIQbf6182bde89f0c7a-partinfo-00000000-QINU UNIQbf6182bde89f0c7a-partinfo-00000001-QINU