Difference between revisions of "Part:BBa K4160002:Design"

 
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===Design Notes===
 
===Design Notes===
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Mutation of GAC to GAT (bp 12) to make it RCF[1000] compatible
  
  

Latest revision as of 19:14, 10 October 2022


IL-6RB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 531
    Illegal PstI site found at 692
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 531
    Illegal PstI site found at 692
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 9
    Illegal BglII site found at 195
    Illegal BglII site found at 459
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 531
    Illegal PstI site found at 692
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 531
    Illegal PstI site found at 692
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Mutation of GAC to GAT (bp 12) to make it RCF[1000] compatible


Source

Expression vector pLeo619. Sequence was found through Genbank https://www.ncbi.nlm.nih.gov/nuccore/MG437012

References