Difference between revisions of "Part:BBa K4140027"

Revision as of 18:53, 10 October 2022

Permeability device

Part Description

In this composite Lac promoter regulate the expression of phenylalanine permease that is a single, integral membrane transporter that uses the proton motive force to antiport L-phenylalanine and L-tyrosine. And present naturally in Escherichia coli.

Usage

We implement this composite to increase the permeability of cells to phenylalanine by the expression of phenylalanine permease that is a single, integral membrane transporter to improve the sensitivity of our circuit to phenylalanine as shown in figure 1 and figure 2.

Figure 1. (shows the permeability circuit in our E.coli-based system.)

Figure 2. (shows the whole cell-based biosensor.)

Experimental Characterization

This figure shows an experimental characterization of this part as it's validated through gel electrophoresis as it is in lane 7. The run part (ordered from IDT) included Lac promoter - Permease.

Sequence and Features