Difference between revisions of "Part:BBa K4229035"
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The T2 proteins builds together with the T3-protein(BBa_K4229036) the second pore structure beside T1 in the full wiffleball(BBa_K4229048/BBa_K4229049). | The T2 proteins builds together with the T3-protein(BBa_K4229036) the second pore structure beside T1 in the full wiffleball(BBa_K4229048/BBa_K4229049). | ||
[[File:BMCs2.jpg|600px|thumb|left|A:Showing the original HO-Shell found in Haliangium orchaceum. B: showing the full wiffleball made with H-/T1-/T2-/T3-protein. C: minimalwiffleball made just our of the H- and T1-protein. ]] | [[File:BMCs2.jpg|600px|thumb|left|A:Showing the original HO-Shell found in Haliangium orchaceum. B: showing the full wiffleball made with H-/T1-/T2-/T3-protein. C: minimalwiffleball made just our of the H- and T1-protein. ]] | ||
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Revision as of 18:07, 10 October 2022
T2 protein from the full wiffleball of the BMC
The T2 proteins builds together with the T3-protein(BBa_K4229036) the second pore structure beside T1 in the full wiffleball(BBa_K4229048/BBa_K4229049).
The plasmid with this protein was sent kindly send to us by the Kerfeld Group after we found this very interesting paper:
H. Kirst and C. A. Kerfeld, “Bacterial microcompartments: Catalysis-enhancing metabolic modules for next-generation metabolic and biomedical engineering,” BMC Biol., vol. 17, no. 1, pp. 1–11, 2019, DOI: 10.1186/s12915-019-0691-z.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 34
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 34
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 34
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 34
Illegal AgeI site found at 337 - 1000COMPATIBLE WITH RFC[1000]