Difference between revisions of "Part:BBa K4165131"
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cbeta_deviations = 0, clashscore = 0, molprobity = 2.71, ramachandran_favored = 0, ramachandran_outliers = 0, Qmean_4 = -6.45183, Qmean6 = -5.51397
 
cbeta_deviations = 0, clashscore = 0, molprobity = 2.71, ramachandran_favored = 0, ramachandran_outliers = 0, Qmean_4 = -6.45183, Qmean6 = -5.51397
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===Modeling===
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<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/htra1-bp/h2k.jpg" style="margin-left:200px;" alt="" width="500" /></p>
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</html>
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                  Figure 1.: 3D Model of H2K peptide Modelled by AlphaFold2.0 displayed in Pyomol
  
 
===References===
 
===References===

Revision as of 17:35, 10 October 2022


HTRA Binding Peptide 37

part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein

Usage and Biology

Synthetic peptide acquired by Phage display, Binds specifically to the water-sites of PDZ domain of HTRA1 Protein with IC50 of 2.0 ± 0.1 and G of 100

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

Got score of 4 out of 6 in our Quality assessment code after modeling by AlphaFold2.0

cbeta_deviations = 0, clashscore = 0, molprobity = 2.71, ramachandran_favored = 0, ramachandran_outliers = 0, Qmean_4 = -6.45183, Qmean6 = -5.51397

Modeling


                 Figure 1.: 3D Model of H2K peptide Modelled by AlphaFold2.0 displayed in Pyomol

References

Beuming, T., Farid, R., & Sherman, W. (2009). High‐energy water sites determine peptide binding affinity and specificity of PDZ domains. Protein Science, 18(8), 1609-1619.