Difference between revisions of "Part:BBa K4461512:Experience"

(→‎Applications of BBa_K4461512)
(→‎Applications of BBa_K4461512)
 
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===Applications of BBa_K4461512===
 
===Applications of BBa_K4461512===
 
We used enzyme digestion and ligation to ligate three devices and pSB1C3. To check if we insert successfully, we use those primers used to add suffix and prefix for them to clone three promoters respectively. The nirBDC promoter with suffix and prefix should be 357bp, and the glpABC promoter and hchA promoter should be 312bp and 261bp.
 
We used enzyme digestion and ligation to ligate three devices and pSB1C3. To check if we insert successfully, we use those primers used to add suffix and prefix for them to clone three promoters respectively. The nirBDC promoter with suffix and prefix should be 357bp, and the glpABC promoter and hchA promoter should be 312bp and 261bp.
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[[File:Ligation promoter test.jpg|400px|thumb|center|Fig1. The result shows that three promoters are in this construct.]]
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[[File:Ligation promoter test.jpg|300px|thumb|center|Fig1. The result shows that three promoters are in this construct.]]
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Then, the FP intensity and OD are measured three times, Fig.2 shows the result.
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[[File:Ligation FP+OD test.png|300px|thumb|center|Fig.2]]
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 17:19, 10 October 2022


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Applications of BBa_K4461512

We used enzyme digestion and ligation to ligate three devices and pSB1C3. To check if we insert successfully, we use those primers used to add suffix and prefix for them to clone three promoters respectively. The nirBDC promoter with suffix and prefix should be 357bp, and the glpABC promoter and hchA promoter should be 312bp and 261bp.

Fig1. The result shows that three promoters are in this construct.

Then, the FP intensity and OD are measured three times, Fig.2 shows the result.

Fig.2

User Reviews

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