Difference between revisions of "Part:BBa K4195069"
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===Biology=== | ===Biology=== | ||
This sequence is a conserved region of toxin gene <i>pirA</i>(<i>1</i>). It’s used as the detection target of RENDR system. | This sequence is a conserved region of toxin gene <i>pirA</i>(<i>1</i>). It’s used as the detection target of RENDR system. | ||
| + | <br> | ||
<b>Ribozyme ENabled Detection of RNA (RENDR)</b> | <b>Ribozyme ENabled Detection of RNA (RENDR)</b> | ||
<br> | <br> | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
| − | ===Usage and | + | ===Usage and Design=== |
| + | <br> | ||
| + | This part is used as the target of the RENDR detection system. For toxin <i>pirA</i>, we designed <partinfo>BBa_K4195141</partinfo>, <partinfo>BBa_K4195142</partinfo>, <partinfo>BBa_K4195145</partinfo>, <partinfo>BBa_K4195146</partinfo>, <partinfo>BBa_K4195156</partinfo>, <partinfo>BBa_K4195157</partinfo>, <partinfo>BBa_K4195160</partinfo>, <partinfo>BBa_K4195161</partinfo>, <partinfo>BBa_K4195168</partinfo>, <partinfo>BBa_K4195169</partinfo>, <partinfo>BBa_K4195172</partinfo>, <partinfo>BBa_K4195173</partinfo>. Other related parts are as followings: <partinfo>BBa_K4195151</partinfo>, <partinfo>BBa_K4195183</partinfo>, <partinfo>BBa_K4195184</partinfo>, <partinfo>BBa_K4195185</partinfo>, <partinfo>BBa_K4195186</partinfo>. | ||
| + | |||
| + | ===Reference=== | ||
| + | <br/> | ||
| + | [1] Lazarte, J., Kim, Y. R., Lee, J. S., Chun, J. H., Kim, S. W., Jung, J. W., Kim, J., Kayansamruaj, P., Thompson, K. D., Kim, H., & Jung, T. S. (2021). Passive Immunization with Recombinant Antibody VLRB-PirA<sup>vp</sup>/PirB<sup>vp</sup>-Enriched Feeds against ''Vibrio parahaemolyticus'' Infection in ''Litopenaeus'' ''vannamei'' Shrimp. ''Vaccines'', 9(1), 55. https://doi.org/10.3390/vaccines9010055 | ||
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Revision as of 16:41, 10 October 2022
pirA_i
Biology
This sequence is a conserved region of toxin gene pirA(1). It’s used as the detection target of RENDR system.
Ribozyme ENabled Detection of RNA (RENDR)
RENDR is a high-performing, plug-and-play RNA-sensing platform(1). RENDR utilizes a split variant of the Tetrahymena thermophila ribozyme by synthetically splitting it into two non-functional fragments (Fig. 1). Two fragments are each appended with designed RNA guide sequences, which can interact with the RNA input of interest. The split ribozyme is then inserted within a desired gene output. When bound with the RNA input, two transcribed split ribozyme fragments are triggered to self-splice and thus the intact transcript of the protein output will form.
Fig. 1 Schematic illustration of RENDR.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]