Difference between revisions of "Part:BBa K4221021"

Latest revision as of 16:11, 10 October 2022

mPETase-GSlinker

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 783
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 783
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 783
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 783
Illegal NgoMIV site found at 58
Illegal NgoMIV site found at 112
Illegal NgoMIV site found at 139
1000
COMPATIBLE WITH RFC[1000]

Usage

PET hydrolase (PETase), which hydrolyzes polyethylene terephthalate (PET) into soluble building blocks, provides an attractive avenue for the bioconversion of plastics.

Biology

PETase is a plastic degrading enzyme derived from Ideonella sakaiensis, mPETase is a complicated mutation of PETase, which contains 11 mutation sites: S214H-I168R-W159H-S188Q-R280A-A180I-G165A-Q119Y-L117F-T140D-S121E.

Design Consideration

The construction includes: BslA with a TEV linker（GAAAACCTGTACTTCCAGGGTTCTGGT）

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K4221021 short</partinfo>
-<!-- Add more about the biology of this part here
-===Usage and Biology===
 <!-- -->
@@ Line 18: / Line 12: @@
 <partinfo>BBa_K4221021 parameters</partinfo>
 <!-- -->
+===Usage===
+PET hydrolase (PETase), which hydrolyzes polyethylene terephthalate (PET) into soluble building blocks, provides an attractive avenue for the bioconversion of plastics.
+===Biology===
+PETase is a plastic degrading enzyme derived from Ideonella sakaiensis, mPETase is a complicated mutation of PETase, which contains 11 mutation sites: S214H-I168R-W159H-S188Q-R280A-A180I-G165A-Q119Y-L117F-T140D-S121E.
+===Design Consideration===
+The construction includes:
+BslA with a TEV linker（GAAAACCTGTACTTCCAGGGTTCTGGT）
+<!-- Add more about the biology of this part here
+===Usage and Biology===