Difference between revisions of "Part:BBa K4187020"
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<partinfo>BBa_K4187020 short</partinfo> | <partinfo>BBa_K4187020 short</partinfo> | ||
| − | . | + | EL222 is a natural photosensitive DNA-binding protein from the marine bacterium Erythrobacter litoralis HTCC2594. In the dark, EL222 is in its inactive form where its N-terminal LOV domain represses its DNA-binding C-terminal HTH domain. Upon blue light exposure (450nm), LOV-HTH interaction is released, allowing it to dimerize and bind its DNA binding regions. In our system, this protein will regulate the transcription of the PLA depolymerase (BBa_K4187021) |
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Latest revision as of 15:43, 10 October 2022
T7-RBS-EL222-T1-T7TE
EL222 is a natural photosensitive DNA-binding protein from the marine bacterium Erythrobacter litoralis HTCC2594. In the dark, EL222 is in its inactive form where its N-terminal LOV domain represses its DNA-binding C-terminal HTH domain. Upon blue light exposure (450nm), LOV-HTH interaction is released, allowing it to dimerize and bind its DNA binding regions. In our system, this protein will regulate the transcription of the PLA depolymerase (BBa_K4187021)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 563
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 133
- 1000COMPATIBLE WITH RFC[1000]