Difference between revisions of "Part:BBa K2205000"
Line 8: Line 8:
 
Due to time constraints, work on assembling and further characterising this Biobrick was unable to finish.
 
Due to time constraints, work on assembling and further characterising this Biobrick was unable to finish.
  
−
<html><a href="https://2022.igem.wiki/tu-dresden/">TU_Dresden 22 team</a> suggests to use this part could use as an <b>alternative</b> to <a href="https://parts.igem.org/Part:BBa_J04450">BBa_J04450</a></html> for white-red colony selection.
+
This part could be used as an <b>alternative</b> to <html><a href="https://parts.igem.org/Part:BBa_J04450">BBa_J04450</a> for white-red colony selection as suggested by <a href="https://2022.igem.wiki/tu-dresden/">TU_Dresden22 team.</a></html>
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 15:24, 10 October 2022


J23100-RFP

BBa_K2205000 is a proposed composite BioBrick reporter that constitutively produces MFRP1. It possesses an MRFP1 coding sequence (E1010) after a strong Anderson promoter (J23100) and RBS (B0034). A double terminator (B0015) was added after the MRFP1 sequence. A BioBrick RFC10 prefix and suffix were added as well.

The construct was submitted to IDT for synthesis as a gBlock. Due to time constraints, work on assembling and further characterising this Biobrick was unable to finish.

This part could be used as an alternative to BBa_J04450 for white-red colony selection as suggested by TU_Dresden22 team.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 616
    Illegal AgeI site found at 728
  • 1000
    COMPATIBLE WITH RFC[1000]

Part Documentation by TU_Dresden 2022 Team

Table of content
  1. Biology
  2. Expression
  3. Comparison to constitutative promoter
  4. Item4



  1. Biology
  2. Expression
  3. Comparison to constitutative promoter
  4. Item4

Selection of constitutive promotor

Comparison with BBa_J04450

Part was compared to BBa_J04450.

Figure 1: A. Lac promoter of BBa_J04450 part and mRFP1 reporter downstream in presence of inductor (IPTG) and in its absence. B. Fluorescence/absorbance 600nm of mRFP1 of the BBa_J04450 compared to BBa_K2205000 part with J23100 promoter over 24 hour incubation of bacterial culture.


Usage and Biology

TEst